Optimization of media constituents for the production of lipase in solid state fermentation by Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)
نویسندگان
چکیده
The production of extra cellular lipase in Solid State Fermentation (SSF) using Yarrowia lipolytica NCIM 3589 with Palm Kernal cake (Elaeis guineensis) has been studied. Different parameters such as incubation time, inoculum level, initial moisture content, carbon level and nitrogen level of the medium were optimized. Screening of various process variables has been accomplished with the help of Plackett-Burman design. The maximum lipase activity of 18.58 units per gram of dry fermented substrate (U/gds) was observed with the substrate of Palm Kernal cake in four days of fermentation.
منابع مشابه
Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus)
Mustard oil cake (Brassica napus), the residue obtained after extraction of mustard oil from mustard oil seeds, was investigated for the production of lipase under solid state fermentation (SSF) using the marine yeast Yarrowia lipolytica NCIM 3589. Process parameters such as incubation time, biomass concentration, initial moisture content, carbon source concentration and nitrogen source concent...
متن کاملMolecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica
Diacylglycerol acyltransferases (DGAT) are involved in the acylation of sn-1,2-diacylglycerol. Palm kernel oil, extracted from Elaeis guineensis (oil palm) seeds, has a high content of medium-chain fatty acids mainly lauric acid (C12:0). A putative E. guineensis diacylglycerol acyltransferase gene (EgDGAT1-1) is expressed at the onset of lauric acid accumulation in the seed endosperm suggesting...
متن کاملThe production of Yarrowia lipolytica lipase powder by improved spray-drying method
Lipase is used in the production of foods, flavor enhancers, detergents, cosmetics and pharmaceuticals. A common impediment to the production of commercial enzymes is their low-stability aqueous solutions. In this study, the downstream process was investigated to obtain a stable spray-dried lipase powder of Yarrowia lipolytica. The enzyme solution samples were supplemented with different concen...
متن کاملQuality Characteristics of Chevon Sausage Obtained from Goats Fed a 50% Inclusion Level of Melon (Colocynthis citrillus) Husk and Palm (Elaeis guineensis) Oil Slurry
A study was undertaken to determine effect of nutritional value of Melon husk (MH) and Palm oil slurry (POS) on quality of chevon obtained from West African Dwarf (WAD) goats fed finishing diets as follows: diet 1(0% MH, 0% POS); diet 2 (50% MH, 0% POS); diet 3 (0% MH, 50% POS) and diet 4 (50% MH, 50% POS), along with Panicum maximum as basal diet for all dietary treatment groups. After 60 days...
متن کاملLipase Production in Solid State Fermentation Using Aspergillus niger: Response Surface Methodology
Among enzymes, lipases have been widely investigated because of the numerous industrial applications. In this study, optimization of lipase production by Aspergillus niger in solid state fermentation from rice bran as solid substrate was investigated. The optimal conditions with the aid of central composite design (CCD) under response surface methodology (RSM) were obtained. In the analysis of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010